mRNA Core

Goal

Our goal is to advance mRNA-LNP vaccine and therapeutic research by providing reliable and high-quality mRNA and LNP products that support research from early discovery through preclinical evaluation.

Services Offered

·     UTR and codon-optimization of genes of interest to maximize mRNA expression and potency.

·     Small- and large-scale production of sequence-optimized in vitro transcribed (IVT) mRNA.

·     Off-the-shelf IVT mRNAs encoding commonly requested genes, including reporters such as mCherry, Luciferase, GFP, Ovalbumin, IL-12, and Cas9.

·     mRNA encapsulation into lipid nanoparticles (LNPs), primarily using SM-102 and ALC-315. Other non-proprietary lipids may be considered on a case-by-case basis.

·     Off-the-shelf LNPs such as empty LNPs and commonly requested reporters such as Luc, GFP and mCherry.

·     High-throughput LNP formulation services, including evaluation of lipid ratios, different mRNA sequences, individual lipids, and lipid combinations.

·     Surface modification of mRNA-LNPs to facilitate targeted delivery to specific tissues and cell types.

·     Fluorescent labeling of mRNA (such as Cy 5.5), LNPs (such as DiL, DiO, DiI), and mRNA-LNPs (dual labelling) for nanoparticle tracking, biodistribution, and microscopy.

·     LNP characterization including particle size, polydispersity (PDI), particle count, and mRNA encapsulation efficiency.

Please note that all orders are placed and processed through iLab. Custom mRNAs and LNPs, along with off-the-shelf products such as reporter mRNA and reporter mRNA-LNPs, are available for direct ordering through this link.

Pricing 

Internal charges (University of Pennsylvania and Children’s Hospital of Philadelphia only):

• Consultation to aid construction design, pricing information or experiment planning: complimentary
• IVT mRNA: $1500/mg
  • Minimum order: 100ug
• LNPs: $1000/mg
  • Minimum order: 100ug for off-the-shelf mRNA-LNP and 200ug for custom mRNA-LNP

External charges (Academic and corporate):

• Consultation to aid construction design, pricing information or experiment planning: complimentary
• IVT mRNA: $2500/mg
  • Minimum order: 100ug
• LNPs: $1625/mg
  • Minimum order: 100ug for off-shelf mRNA-LNP and 500ug for custom mRNA-LNP

Contact Information

To explore how the Engineered mRNA and Targeted Nanomedicine core might be helpful to your project, or for pricing/quote, please contact Dr. Garima Dwivedi and Dr. Benjamin Davis to schedule an initial consultation.

Garima Dwivedi

Senior Research Investigator, Weissman Lab
Director, mRNA Core, Penn Institute of RNA innovation
4012, 1UCity Sq
25 N 38^th St
Philadelphia PA 19104

Email: garima.dwivedi@pennmedicine.upenn.edu

Benjamin Davis

User Facility Director, AIRFoundry
4072, 1UCity Sq
25 N 38^th St
Philadelphia PA 19104

Email: benjamin.davis@pennmedicine.upenn.edu

AIRFoundry